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CEP68 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CEP68 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113411-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CEP68 (NM_015147), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CEP68 | KIAA0582 | Centrosomal protein 68kDa | Centrosomal protein of 68 kDa
Origin Species
Human
NCBI GenBank
NM_015147

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.07 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALGEEKAEA EASEDTKAQS YGRGSCRERE LDIPGPMSGE QPPRLEAEGG LISPVWGAEG
61IPAPTCWIGT DPGSPSRAHQ PQASDANREP VAERSEPALS GLPPATMGSG DLLLSGESQV
121EKTKLSSSEE FPQTLSLPRT TTICSGHDAD TEDDPSLADL PQALDLSQQP HSSGLSCLSQ
181WKSVLSPGSA AQPSSCSISA SSTGSSLQGH QERAEPRGGS LAKVSSSLEP VVPQEPSSVV
241GLGPRPQWSP QPVFSGGDAS GLGRRRLSFQ AEYWACVLPD SLPPSPDRHS PLWNPNKEYE
301DLLDYTYPLR PGPQLPKHLD SRVPADPVLQ DSGVDLDSFS VSPASTLKSP TNVSPNCPPA
361EATALPFSGP REPSLKQWPS RVPQKQGGMG LASWSQLAST PRAPGSRDAR WERREPALRG
421AKDRLTIGKH LDMGSPQLRT RDRGWPSPRP EREKRTSQSA RRPTCTESRW KSEEEVESDD
481EYLALPARLT QTFCCQLEEL ICWLYNVADV TDHGTAARSN LTSLKSSLQL YRQFKKDIDE
541HQSLTESVLQ KGEILLQCLL ENTPVLEDVL GRIAKQSGEL ESHADRLYDS ILASLDMLAG
601CTLIPDKKPM AAMEHPCEGV TRTRPLEQKL ISEEDLAAND ILDYKDDDDK V

651 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.