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CEP57L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CEP57L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105171-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C6ORF182(CEP57L1) (NM_173830), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C6orf182 | Cep57R | Centrosomal protein CEP57L1 | Cep57-related protein | CEP57L1 | BA487F23.2
Origin Species
Human
NCBI GenBank
AK027189
SwissProt
Q8IYX8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MDSELMHSIV GSYHKPPERV FVPSFTQNEP SQNCHPANLE VTSPKILHSP NSQALILALK
61TLQEKIHRLE LERTQAEDNL NILSREAAQY KKALENETNE RNLAHQELIK QKKDISIQLS
121SAQSRCTLLE KQLEYTKRMV LNVEREKNMI LEQQAQLQRE KEQDQMKLYA KLEKLDVLEK
181ECFRLTTTQK TAEDKIKHLE EKLKEEEHQR KLFQDKASEL QTGLEISKII MSSVSNLKHS
241KEKKKSSKKT KCIKRRPPWQ ICSKFGALPF VAEKMRQHRD PHILQKPFNV TETRCLPKPS
301RTTSWCKAIP PDSEKSISIC DNLSELLMAM QDELDQMSME HQELLKQMKE TESHSVCDDI
361ECELECLLKK MEIKGEQISK LKKHQDSVCK LQQKVQNSKM SEASGIQQED SYPKGSKNIK
421NSPRKCLTDT NLFQKNSSFH PIRVHNLQMK LRRDDIMWEQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

460 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.