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CEP55 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CEP55 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CEP55 antibodies →
$504.00each
Catalog Number: LS-G124549-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CEP55 (NM_001127182), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 111 | Centrosomal protein of 55 kDa | C10orf3 | CT111 | Centrosomal protein 55kDa | URCC6 | CEP55 | Up-regulated in colon cancer 6
Origin Species
Human
NCBI GenBank
NM_018131
SwissProt
Q53EZ4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54 kda
Protein Type
Lysate
Amino Acid Sequence
1MSSRSTKDLI KSKWGSKPSN SKSETTLEKL KGEIAHLKTS VDEITSGKGK LTDKERHRLL
61EKIRVLEAEK EKNAYQLTEK DKEIQRLRDQ LKARYSTTAL LEQLEETTRE GERREQVLKA
121LSEEKDVLKQ QLSAATSRIA ELESKTNTLR LSQTVAPNCF NSSINNIHEM EIQLKDALEK
181NQQWLVYDQQ REVYVKGLLA KIFELEKKTE TAAHSLPQQT KKPESEGYLQ EEKQKCYNDL
241LASAKKDLEV ERQTITQLSF ELSEFRRKYE ETQKEVHNLN QLLYSQRRAD VQHLEDDRHK
301TEKIQKLREE NDIARGKLEE EKKRSEELLS QVQFLYTSLL KQQEEQTRVA LLEQQMQACT
361LDFENEKLDR QHVQHQLLVI LKELRKARNQ ITQLESLKQL HEFAITEPLV TFQGETENRE
421KVAASPKSPT AALNESLVEC PKCNIQYPAT EHRDLLVHVE YCSK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

464 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.