Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CEP44 / KIAA1712 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126643-100
Catalog Number: LS-G126643
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1712(CEP44) (NM_001145314), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CEP44 | Centrosomal protein 44kDa | Centrosomal protein of 44 kDa | KIAA1712 | PS1TP3
- Origin Species
- Human
- NCBI GenBank
- NM_001040157
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATGDLKRSL RNLEQVLRLL NYPEEVDCVG LIKGDPAASL PIISYSFTSY SPYVTELIME61SNVELIAKND LRFIDAVYKL LRDQFNYKPI LTKKQFIQCG FAEWKIQIVC DILNCVMKKH121KELSSLQKIP SQQRKKISSG KSEPPLGNEK ISAEAVGVDI SGRFMTSGKK KAVVIRHLYN181EDNVDISEDT LSPITDVNEA VDVSDLNATE IKMPEVKVPE IKAEQQDVNV NPEITALQTM241LAECQENLKK LTSIEKRLDC LEQKMKGKVM VDENTWTNLL SRVTLLETEM LLSKKNDEFI301EFNEVSEDYA SCSDMDLLNP HRKSEVERPA SIPLSSGYST ASSDSTPRAS TVNYCGLNEI361SENFPAWSAT SSCSSLSWLL RGHTSYLSSQ SFAWPLSCVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
399 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.