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CEP104 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CEP104 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CEP104 antibodies →
$725.00each
Catalog Number: LS-G113748-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CEP104 (NM_014704), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Centrosomal protein 104kDa | Centrosomal protein of 104 kDa | CEP104 | KIAA0562 | RP1-286D6.4 | GlyBP
Origin Species
Human
NCBI GenBank
NM_014704
SwissProt
O60308

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
104.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MPHKIGFVVV SSSGHEDGFS ARELMIHAPT VSGWRSPRFC QFPQEIVLQM VERCRIRKLQ
61LLAHQYMISS KIEFYISESL PEYFAPYQAE RFRRLGYVSL CDNEKTGCKA RELKSVYVDA
121VGQFLKLIFH QNHVNKYNIY NQVALVAINI IGDPADFSDE SNTASREKLI DHYLGHNSED
181PALEGTYARK SDYISPLDDL AFDMYQDPEV AQIIRKLDER KREAVQKERY DYAKKLKQAI
241ADLQKVGERL GRYEVEKRCA VEKEDYDLAK EKKQQMEQYR AEVYEQLELH SLLDAELMRR
301PFDLPLQPLA RSGSPCHQKP MPSLPQLEER GTENQFAEPF LQEKPSSYSL TISPQHSAVD
361PLLPATDPHP KINAESLPYD ERPLPAIRKH YGEAVVEPEM SNADISDARR GGMLGEPEPL
421TEKALREASS AIDVLGETLV AEAYCKTWSY REDALLALSK KLMEMPVGTP KEDLKNTLRA
481SVFLVRRAIK DIVTSVFQAS LKLLKMIITQ YIPKHKLSKL ETAHCVERTI PVLLTRTGDS
541SARLRVTAAN FIQEMALFKE VKSLQIIPSY LVQPLKANSS VHLAMSQMGL LARLLKDLGT
601GSSGFTIDNV MKFSVSALEH RVYEVRETAV RIILDMYRQH QASILEYLPP DDSNTRRNIL
661YKTIFEGFAK IDGRATDAEM RARRKAATEE AEKQKKEEIK ALQGQLAALK EIQAEVQEKE
721SDAVKPKNQD IQGGKAAPAE ALGIPDEHYL DNLCIFCGER SESFTEEGLD LHYWKHCLML
781TRCDHCKQVV EISSLTEHLL TECDKKDGFG KCYRCSEAVF KEELPRHIKH KDCNPAKPEK
841LANRCPLCHE NFSPGEEAWK AHLMGPAGCT MNLRKTHILQ KAPALQPGKS SAVAASGPLG
901SKAGSKIPTP KGGLSKSSSR TYAKR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

925 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.