Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CENTG2 / AGAP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101272-100
Catalog Number: LS-G101272
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AGAP1 (NM_014914), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AGAP-1 | AGAP1 | Centaurin | gamma 2 | CENTG2 | Cnt-g2 | KIAA1099 | GGAP1 | Centaurin-gamma-2
- Origin Species
- Human
- NCBI GenBank
- NM_014914
- SwissProt
- Q9UPQ3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNYQQQLANS AAIRAEIQRF ESVHPNIYSI YELLERVEEP VLQNQIREHV IAIEDAFVNS61QEWTLSRSVP ELKVGIVGNL ASGKSALVHR YLTGTYVQEE SPEGGRFKKE IVVDGQSYLL121LIRDEGGPPE AQFAMWVDAV IFVFSLEDEI SFQTVYHYYS RMANYRNTSE IPLVLVGTQD181AISSANPRVI DDARARKLSN DLKRCTYYET CATYGLNVER VFQDVAQKIV ATRKKQQLSI241GPCKSLPNSP SHSSVCSAQV SAVHISQTSN GGGSLSDYSS SVPSTPSTSQ KELRIDVPPT301ANTPTPVRKQ SKRRSNLFTS RKGSDPDKEK KGLESRADSI GSGRAIPIKQ GMLLKRSGKS361LNKEWKKKYV TLCDNGVLTY HPSLHDYMQN VHGKEIDLLR TTVKVPGKRP PRATSACAPI421SSPKTNGLSK DMSSLHISPN SDTGLGDSVC SSPSISSTTS PKLDPPPSPH ANRKKHRRKK481STSNFKADGL SGTAEEQEEN FEFIIVSLTG QTWHFEATTY EERDAWVQAI ESQILASLQS541CESSKNKSRL TSQSEAMALQ SIRNMRGNSH CVDCETQNPN WASLNLGALM CIECSGIHRN601LGTHLSRVRS LDLDDWPVEL IKVMSSIGNE LANSVWEESS QGRTKPSVDS TREEKERWIR661AKYEQKLFLA PLPCTELSLG QHLLRATADE DLRTAILLLA HGSRDEVNET CGEGDGRTAL721HLACRKGNVV LAQLLIWYGV DVTARDAHGN TALAYARQAS SQECIDVLLQ YGCPDERFVL781MATPNLSRRN NNRNNSSGRV PTIITRTRPLEQKLISEEDLAANDILDYKDDDDKV
804 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.