Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CENPC / CENP-C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118386-100
Catalog Number: LS-G118386
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CENPC (NM_001812), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CENP-C 1 | CENPC1 | Centromere autoantigen C | Centromere protein C 1 | CENP-C | Centromere autoantigen C1 | Centromere protein c | Hcp-4 | MIF2 | ICEN7 | CENPC
- Origin Species
- Human
- NCBI GenBank
- NM_001812
- SwissProt
- Q03188
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 106.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAASGLDHLK NGYRRRFCRP SRARDINTEQ GQNVLEILQD CFEEKSLAND FSTNSTKSVP61NSTRKIKDTC IQSPSKECQK SHPKSVPVSS KKKEASLQFV VEPSEATNRS VQAHEVHQKI121LATDVSSKNT PDSKKISSRN INDHHSEADE EFYLSVGSPS VLLDAKTSVS QNVIPSSAQK181RETYTFENSV NMLPSSTEVS VKTKKRLNFD DKVMLKKIEI DNKVSDEEDK TSEGQERKPS241GSSQNRIRDS EYEIQRQAKK SFSTLFLETV KRKSESSPIV RHAATAPPHS CPPDDTKLIE301DEFIIDESDQ SFASRSWITI PRKAGSLKQR TISPAESTAL FQGRKSREKH HNILPKTLAN361DKHSHKPHPV ETSQPSDKTV LDTSYALIGE TVNNYRSTKY EMYSKNAEKP SRSKRTIKQK421QRRKFMAKPA EEQLDVGQSK DENIHTSHIT QDEFQRNSDR NMEEHEEMGN DCVSKKQMPP481VGSKKSSTRK DKEESKKKRF SSESKNKLVP EEVTSTVTKS RRISRRPSDW WVVKSEESPV541YSNSSVRNEL PMHHNSSRKS TKKTNQSSKN IRKKTIPLKR QKTATKGNQR VQKFLNAEGS601GGIVGHDEIS RCSLSEPLES DEADLAKKKN LDCSRSTRSS KNEDNIMTAQ NVPLKPQTSG661YTCNIPTESN LDSGEHKTSV LEESGPSRLN NNYLMSGKND VDDEEVHGSS DDSKQSKVIP721KNRIHHKLVL PSNTPNVRRT KRTRLKPLEY WRGERIDYQG RPSGGFVISG VLSPDTISSK781RKAKENIGKV NKKSNKKRIC LDNDERKTNL MVNLGIPLGD PLQPTRVKDP ETREIILMDL841VRPQDTYQFF VKHGELKVYK TLDTPFFSTG KLILGPQEEK GKQHVGQDIL VFYVNFGDLL901CTLHETPYIL STGDSFYVPS GNYYNIKNLR NEESVLLFTQ IKRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
943 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.