Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CELF1 / CUGBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129943-100
Catalog Number: LS-G129943
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CUG BP1(CELF1) (NM_001172639), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Bruno-like protein 2 | BRUNOL2 | Bruno-like 2 | CELF-1 | CUG RNA-binding protein | CUGBP1 | CUG-BP | EDEN-BP | HNab50 | NAB50 | NAPOR | RNA-binding protein BRUNOL-2 | CELF1 | CUG-BP1 | CUGBP | Deadenylation factor CUG-BP | EDEN-BP homolog
- Origin Species
- Human
- NCBI GenBank
- NM_006560
- SwissProt
- Q92879
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAFKLDFLP EMMVDHCSLN SSPVSKKMNG TLDHPDQPDL DAIKMFVGQV PRTWSEKDLR61ELFEQYGAVY EINVLRDRSQ NPPQSKGCCF VTFYTRKAAL EAQNALHNMK VLPGMHHPIQ121MKPADSEKNN VEDRKLFIGM ISKKCTENDI RVMFSSFGQI EECRILRGPD GLSRGCAFVT181FTTRAMAQTA IKAMHQAQTM EGCSSPMVVK FADTQKDKEQ KRMAQQLQQQ MQQISAASVW241GNLAGLNTLG PQYLALYLQL LQQTASSGNL NTLSSLHPMG GLNAMQLQNL AALAAAASAA301QNTPSGTNAL TTSSSPLSVL TSSGSSPSSS SSNSVNPIAS LGALQTLAGA TAGLNVGSLA361GMAALNGGLG SSGLSNGTGS TMEALTQAYS GIQQYAAAAL PTLYNQNLLT QQSIGAAGSQ421KEGPEGANLF IYHLPQEFGD QDLLQMFMPF GNVVSAKVFI DKQTNLSKCF GFVSYDNPVS481AQAAIQSMNG FQIGMKRLKV QLKRSKNDSK PYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
512 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.