Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CEAL2 / CEACAM16 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120579-100
Catalog Number: LS-G120579
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CEACAM16 (NM_001039213), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CEACAM16 | DFNA4B | CEAL2
- Origin Species
- Human
- SwissProt
- Q2WEN9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.68 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDLLSIYSA PVVVSTVLHM LQIRKLRLGR VHKLPRVLEV GNSGTMSRAY TFFFLRWSFM61ALTGYSWLLL SATFLNVGAE ISITLEPAQP SEGDNVTLVV HGLSGELLAY SWYAGPTLSV121SYLVASYIVS TGDETPGPAH TGREAVRPDG SLDIQGILPR HSGTYILQTF NRQLQTEVGY181GHVQVHEILA QPTVLANSTA LVERRDTLRL MCSSPSPTAE VRWFFNGGAL PVALRLGLSP241DGRVLARHGI RREEAGAYQC EVWNPVSVSR SEPINLTVYF GPERVAILQD STTRTGCTIK301VDFNTSLTLW CVSRSCPEPE YVWTFNGQAL KNGQDHLNIS SMTAAQEGTY TCIAKNTKTL361LSGSASVVVK LSAAAVATMI VPVPTKPTEG QDVTLTVQGY PKDLLVYAWY RGPASEPNRL421LSQLPSGTWI AGPAHTGREV GFPNCSLLVQ KLNLTDTGRY TLKTVTVQGK TETLEVELQV481APLGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
484 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.