Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CEACAM8 / CD66b Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118377-100
Catalog Number: LS-G118377
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CD66b(CEACAM8) (NM_001816), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD66b antigen | Carcinoembryonic antigen CGM6 | CEACAM8 | CD67 | CD67 antigen | CGM6 | NCA-95 | CD66b
- Origin Species
- Human
- NCBI GenBank
- NM_001816
- SwissProt
- P31997
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPISAPSCR WRIPWQGLLL TASLFTFWNP PTTAQLTIEA VPSNAAEGKE VLLLVHNLPQ61DPRGYNWYKG ETVDANRRII GYVISNQQIT PGPAYSNRET IYPNASLLMR NVTRNDTGSY121TLQVIKLNLM SEEVTGQFSV HPETPKPSIS SNNSNPVEDK DAVAFTCEPE TQNTTYLWWV181NGQSLPVSPR LQLSNGNRTL TLLSVTRNDV GPYECEIQNP ASANFSDPVT LNVLYGPDAP241TISPSDTYYH AGVNLNLSCH AASNPPSQYS WSVNGTFQQY TQKLFIPNIT TKNSGSYACH301TTNSATGRNR TTVRMITVSD ALVQGSSPGL SARATVSIMI GVLARVALITRTRPLEQKLISEEDLAANDILDYKDDDDKV
349 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.