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CEACAM20 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CEACAM20 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CEACAM20 antibodies →
$725.00each
Catalog Number: LS-G118818-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CEACAM20 (NM_001102598), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CEACAM20 | GPAD9366 | UNQ9366
Origin Species
Human
SwissProt
Q6UY09

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54 kda
Protein Type
Lysate
Amino Acid Sequence
1MGPADSWGHH WMGILLSASL CTVWSPPAAA QLTLNANPLD ATQSEDVVLP VFGTPRTPQI
61HGRSRELAKP SIAVSPGTAI EQKDMVTFYC TTKDVNITIH WVSNNLSVVF HERMQLSKDG
121KILTILIVQR EDSGTYQCEA RDALLSQRSD PIFLDVKYGP DPVEIKLESG VASGEVVEVM
181EGSSMTFLAE TKSHPPCAYT WFLLDSILSH TTRTFTIHAV SREHEGLYRC LVSNSATHLS
241SLGTLKVRVL ETLTMPQVVP SSLNLVENAR SVDLTCQTVN QSVNVQWFLS GQPLLPSEHL
301QLSADNRTLI IHGLQRNDTG PYACEVWNWG SRARSEPLEL TINCPQSSSL SSGAIAGIVI
361GILAVIAVAS ELGYFLYIRN ARRPSRKTTE DPSHETSQPI PKEEHPTEPS SESLSPEYCN
421ISQLQGRIRV ELTKLPSASR RGNSFSPWKP PPKPLMPPLR LVSTVPKNME SIYEELVNPE
481PNTYIQINPS V
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

491 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.