Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDY2A / CDY Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116375-100
Catalog Number: LS-G116375
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDY2A (NM_004825), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDY2A | CDY | CDY2
- Origin Species
- Human
- NCBI GenBank
- NM_004825
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASQEFEVEA IVDKRQDKNG NTQYLVRWKG YDKQDDTWEP EQHLMNCEKC VHDFNRRQTE61KQKKLTWTTT SRIFSNNARR RTSRSTKANY SKNSPKTPVT DKHHRSKNCK LFAASKNVRR121KAASTLSDTK NMEIINSTIE TLAPDSPFDH KKTVSGFQKL EKLDPIAADQ QDTVVFKVTE181GKLLRDPLSH PGAEQTGIQN KTQMHPLMSQ MSGSVTASMA TGSATRKGIV VLIDPLAANG241TTDMHTSVPR VKGGQRNITD DSRGQPFIKK MHFTIRLTES AITYRDIVVK KEDGFTQIVL301STRSTEKNAL NTEVIKEMVN ALNSAAADDS KLVLFSAAGS VFCCGLDFGY FVRHLRNDRN361TASLEMVDTI KNFVNTFIQF KKPIVVSVNG PAIGLGASIL PLCDLVWANE KAWFQTPYTT421FGQSPDGCSS ITFPKMMGKA SANEMLIAGR KLTAREACAK GLVSQVFLTG TFTQEVMIQI481KELASYNAIV LEECKALVRC NIKLELEQAN ERECEVLRKI WSSAQGIESM LKYVENKIDE541FTRTRPLEQKLISEEDLAANDILDYKDDDDKV
541 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.