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CDR2L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CDR2L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113824-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDRL2(CDR2L) (NM_014603), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDR2L | HUMPPA | Paraneoplastic 62 kDa antigen | Paraneoplastic antigen
Origin Species
Human
NCBI GenBank
L02867
SwissProt
Q86X02

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRRAAGMEDF SAEEEESWYD QQDLEQDLHL AAELGKTLLE RNKELEGSLQ QMYSTNEEQV
61QEIEYLTKQL DTLRHVNEQH AKVYEQLDLT ARDLELTNHR LVLESKAAQQ KIHGLTETIE
121RLQAQVEELQ AQVEQLRGLE QLRVLREKRE RRRTIHTFPC LKELCTSPRC KDAFRLHSSS
181LELGPRPLEQ ENERLQTLVG ALRSQVSQER QRKERAEREY TAVLQEYSEL ERQLCEMEAC
241RLRVQELEAE LLELQQMKQA KTYLLGPDDH LAEALLAPLT QAPEADDPQP GRGDDLGAQD
301GVSSPAASPG HVVRKSCSDT ALNAIVAKDP ASRHAGNLTL HANSVRKRGM SILREVDEQY
361HALLEKYEEL LSKCRQHGAG VRHAGVQTSR PISRDSSWRD LRGGEEGQGE VKAGEKSLSQ
421HVEAVDKRLE QSQPEYKALF KEIFSRIQKT KADINATKVK THSSK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

465 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.