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CDKL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CDKL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G116811-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDKL1 (NM_004196), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDKL1 | CDC2-related kinase 1 | KKIALRE | p42 | Protein kinase p42 KKIALRE | Cyclin-dependent kinase-like 1
Origin Species
Human
NCBI GenBank
X66358
SwissProt
Q00532

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
41.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMEKYEKIGK IGEGSYGVVF KCRNRDTGQI VAIKKFLESE DDPVIKKIAL REIRMLKQLK
61HPNLVNLLEV FRRKRRLHLV FEYCDHTVLH ELDRYQRGVP EHLVKSITWQ TLQAVNFCHK
121HNCIHRDVKP ENILITKHSV IKLCDFGFAR LLTGPSDYYT DYVATRWYRS PELLVGDTQY
181GPPVDVWAIG CVFAELLSGV PLWPGKSDVD QLYLIRKTLG DLIPRHQQVF STNQYFSGVK
241IPDPEDMEPL ELKFPNISYP ALGLLKGCLH MDPTQRLTCE QLLHHPYFEN IREIEDLAKE
301HNKPTRKTLR KSRKHHCFTE TSKLQYLPQL TGSSILPALD NKKYYCDTKK LNYRFPNI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

358 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.