Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDK20 / CCRK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114634-100
Catalog Number: LS-G114634
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCRK(CDK20) (NM_012119), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAK-kinase p42 | Cell cycle related kinase | Cell cycle-related kinase | CDK20 | Cyclin-dependent kinase 20 | p42 | PNQALRE | CCRK | CDCH | CDK-activating kinase p42
- Origin Species
- Human
- NCBI GenBank
- NM_012119
- SwissProt
- Q8IZL9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDQYCILGRI GEGAHGIVFK AKHVETGEIV ALKKVALRRL EDGFPNQALR EIKALQEMED61NQYVVQLKAV FPHGGGFVLA FEFMLSDLAE VVRHAQRPLA QAQVKSYLQM LLKGVAFCHA121NNIVHRDLKP ANLLISASGQ LKIADFGLAR VFSPDGSRLY THQVATRSVG CIMGELLNGS181PLFPGKNDIE QLCYVLRILG TPNPQVWPEL TELPDYNKIS FKEQVPMPLE EVLPDVSPQA241LDLLGQFLLY PPHQRIAASK ALLHQYFFTA PLPAHPSELP IPQRLGGPAP KAHPGPPHIH301DFHVDRPLEE SLLNPELIRP FILEGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
325 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.