Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDK19 / CDK11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113481-100
Catalog Number: LS-G113481
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDC2L6(CDK19) (NM_015076), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA346C16.3 | CDC2L6 | CDK11 | CDC2-related protein kinase 6 | Cyclin-dependent kinase 19 | CDK19 | Cyclin-dependent kinase 11 | Death-preventing kinase | KIAA1028
- Origin Species
- Human
- NCBI GenBank
- AY028424
- SwissProt
- Q9BWU1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDYDFKAKLA AERERVEDLF EYEGCKVGRG TYGHVYKARR KDGKDEKEYA LKQIEGTGIS61MSACREIALL RELKHPNVIA LQKVFLSHSD RKVWLLFDYA EHDLWHIIKF HRASKANKKP121MQLPRSMVKS LLYQILDGIH YLHANWVLHR DLKPANILVM GEGPERGRVK IADMGFARLF181NSPLKPLADL DPVVVTFWYR APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED241IKTSNPFHHD QLDRIFSVMG FPADKDWEDI RKMPEYPTLQ KDFRRTTYAN SSLIKYMEKH301KVKPDSKVFL LLQKLLTMDP TKRITSEQAL QDPYFQEDPL PTLDVFAGCQ IPYPKREFLN361EDDPEEKGDK NQQQQQNQHQ QPTAPPQQAA APPQAPPPQQ NSTQTNGTAG GAGAGVGGTG421AGLQHSQDSS LNQVPPNKKP RLGPSGANSG GPVMPSDYQH SSSRLNYQSS VQGSSQSQST481LGYSSSSQQS SQYHPSHQAH RYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
502 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.