Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDK11A / CDC2L2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108209-100
Catalog Number: LS-G108209
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDC2L2 (NM_033532), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDK11 | CDK11A | CDC2L3 | Cell division cycle 2-like 3 | Cyclin-dependent kinase 11A | CDK11-p110 | CDK11-p58 | PITSLRE A | PITSLRE B | CDC2L2 | CDK11-p46 | Cell division cycle 2-like 2 | p58GTA | PITSLRE | PITSLRE protein kinase beta | PITSLREB
- Origin Species
- Human
- SwissProt
- Q9UQ88
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKNEKMKTTS WLFQSHGSTE IPGRVKKQRK KWGCRSVEEF QCLNRIEEGT YGVVYRAKDK61KTDEIVALKR LKMEKEKEGF PITSLREINT ILKAQHPNIV TVREIVVGSN MDKIYIVMNY121VEHDLKSLME TMKQPFLPGE VKTLMIQLLR GVKHLHDNWI LHRDLKTSNL LLSHAGILKV181GDFGLAREYG SPLKAYTPVV VTQWYRAPEL LLGAKEYSTA VDMWSVGCIF GELLTQKPLF241PGNSEIDQIN KVFKELGTPS EKIWPGYSEL PVVKKMTFSE HPYNNLRKRF GALLSDQGFD301LMNKFLTYFP GRRISAEDGL KHEYFRETPL PIDPSMFPTW PAKSEQQRVK RGTSPRPPEG361GLGYSQLGDD DLKETGFHLT TTNQGASAAG PGFSLKFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
397 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.