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CDHR2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CDHR2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CDHR2 antibodies →
$725.00each
Catalog Number: LS-G112297-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCLKC(CDHR2) (NM_017675), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH24
Origin Species
Human
NCBI GenBank
NM_017675

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
141.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAQLWLSCFL LPALVVSVAA NVAPKFLANM TSVILPEDLP VGAQAFWLVA EDQDNDPLTY
61GMSGPNAYFF AVTPKTGEVK LASALDYETL YTFKVTISVS DPYIQVQREM LVIVEDRNDN
121APVFQNTAFS TSINETLPVG SVVFSVLAVD KDMGSAGMVV YSIEKVIPST GDSEHLFRIL
181ANGSIVLNGS LSYNNKSAFY QLELKACDLG GMYHNTFTIQ CSLPVFLSIS VVDQPDLDPQ
241FVREFYSASV AEDAAKGTSV LTVEAVDGDK GINDPVIYSI SYSTRPGWFD IGADGVIRVN
301GSLDREQLLE ADEEVQLQVT ATETHLNIYG QEAKVSIWVT VRVMDVNDHK PEFYNCSLPA
361CTFTPEEAQV NFTGYVDEHA SPRIPIDDLT MVVYDPDKGS NGTFLLSLGG PDAEAFSVSP
421ERAAGSASVQ VLVRVSALVD YERQTAMAVQ VVATDSVSQN FSVAMVTIHL RDINDHRPTF
481PQSLYVLTVP EHSATGSVVT DSIHATDPDT GAWGQITYSL LPGNGADLFQ VDPVSGTVTV
541RNGELLDRES QAVYYLTLQA TDGGNLSSST TLQIHLLDIN DNAPVVSGSY NIFVQEEEGN
601VSVTIQAHDN DEPGTNNSRL LFNLLPGPYS HNFSLDPDTG LLRNLGPLDR EAIDPALEGR
661IVLTVLVSDC GEPVLGTKVN VTITVEDIND NLPIFNQSSY NFTVKEEDPG VLVGVVKAWD
721ADQTEANNRI SFSLSGSGAN YFMIRGLVLG AGWAEGYLRL PPDVSLDYET QPVFNLTVSA
781ENPDPQGGET IVDVCVNVKD VNDNPPTLDV ASLRGIRVAE NGSQHGQVAV VVASDVDTSA
841QLEIQLVNIL CTKAGVDVGS LCWGWFSVAA NGSVYINQSK AIDYEACDLV TLVVRACDLA
901TDPGFQAYSN NGSLLITIED VNDNAPYFLP ENKTFVIIPE LVLPNREVAS VRARDDDSGN
961NGVILFSILR VDFISKDGAT IPFQGVFSIF TSSEADVFAG SIQPVTSLDS TLQGTYQVTV
1021QARDRPSLGP FLEATTTLNL FTVDQSYRSR LQFSTPKEEV GANRQAINAA LTQATRTTVY
1081IVDIQDIDSA ARARPHSYLD AYFVFPNGSA LTLDELSVMI RNDQDSLTQL LQLGLVVLGS
1141QESQESDLSK QLISVIIGLG VALLLVLVIM TMAFVCVRKS YNRKLQAMKA AKEARKTAAG
1201VMPSAPAIPG TNMYNTERAN PMLNLPNKDL GLEYLSPSND LDSVSVNSLD DNSVDVDKNS
1261QEIKEHRPPH TPPEPDPEPL SVVLLGRQAG ASGQLEGPSY TNAGLDTTDL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1310 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.