Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDH8 / Cadherin 8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99565-100
Catalog Number: LS-G99565
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cadherin 8(CDH8) (NM_001796), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cadherin 8 | type 2 | CDH8 | Nbla04261 | Cadherin-8
- NCBI GenBank
- NM_001796
- SwissProt
- P55286
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88.25 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPERLAEMLL DLWTPLIILW ITLPPCIYMA PMNQSQVLMS GSPLELNSLG EEQRILNRSK61RGWVWNQMFV LEEFSGPEPI LVGRLHTDLD PGSKKIKYIL SGDGAGTIFQ INDVTGDIHA121IKRLDREEKA EYTLTAQAVD WETSKPLEPP SEFIIKVQDI NDNAPEFLNG PYHATVPEMS181ILGTSVTNVT ATDADDPVYG NSAKLVYSIL EGQPYFSIEP ETAIIKTALP NMDREAKEEY241LVVIQAKDMG GHSGGLSGTT TLTVTLTDVN DNPPKFAQSL YHFSVPEDVV LGTAIGRVKA301NDQDIGENAQ SSYDIIDGDG TALFEITSDA QAQDGIIRLR KPLDFETKKS YTLKVEAANV361HIDPRFSGRG PFKDTATVKI VVEDADEPPV FSSPTYLLEV HENAALNSVI GQVTARDPDI421TSSPIRFSID RHTDLERQFN INADDGKITL ATPLDRELSV WHNITIIATE IRNHSQISRV481PVAIKVLDVN DNAPEFASEY EAFLCENGKP GQVIQTVSAM DKDDPKNGHY FLYSLLPEMV541NNPNFTIKKN EDNSLSILAK HNGFNRQKQE VYLLPIIISD SGNPPLSSTS TLTIRVCGCS601NDGVVQSCNV EAYVLPIGLS MGALIAILAC IILLLVIVVL FVTLRRHKNE PLIIKDDEDV661RENIIRYDDE GGGEEDTEAF DIATLQNPDG INGFLPRKDI KPDLQFMPRQ GLAPVPNGVD721VDEFINVRLH EADNDPTAPP YDSIQIYGYE GRGSVAGSLS SLESTTSDSD QNFDYLSDWG781PRFKRLGELY SVGESDKETTRTRPLEQKLISEEDLAANDILDYKDDDDKV
799 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.