Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDH7 / Cadherin 7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108183-100
Catalog Number: LS-G108183
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cadherin 7(CDH7) (NM_033646), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cadherin-7 | CDH7L1 | CDH7 | Cadherin 7 | type 2
- Origin Species
- Human
- NCBI GenBank
- NM_004361
- SwissProt
- Q9ULB5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 84 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLGKVEFCH FLQLIALFLC FSGMSQAELS RSRSKPYFQS GRSRTKRSWV WNQFFVLEEY61MGSDPLYVGK LHSDVDKGDG SIKYILSGEG ASSIFIIDEN TGDIHATKRL DREEQAYYTL121RAQALDRLTN KPVEPESEFV IKIQDINDNE PKFLDGPYTA GVPEMSPVGT SVVQVTATDA181DDPTYGNSAR VVYSILQGQP YFSVEPKTGV IKTALPNMDR EAKDQYLLVI QAKDMVGQNG241GLSGTTSVTV TLTDVNDNPP RFPRRSYQYN VPESLPVASV VARIKAADAD IGANAEMEYK301IVDGDGLGIF KISVDKETQE GIITIQKELD FEAKTSYTLR IEAANKDADP RFLSLGPFSD361TTTVKIIVED VDEPPVFSSP LYPMEVSEAT QVGNIIGTVA AHDPDSSNSP VRYSIDRNTD421LERYFNIDAN SGVITTAKSL DRETNAIHNI TVLAMESQNP SQVGRGYVAI TILDINDNAP481EFAMDYETTV CENAQPGQVI QKISAVDKDE PSNGHQFYFS LTTDATNNHN FSLKDNKDNT541ASILTRRNGF RRQEQSVYYL PIFIVDSGSP SLSSTNTLTI RVCDCDADGV AQTCNAEAYV601LPAGLSTGAL IAILACVLTL LVLILLIVTM RRRKKEPLIF DEERDIRENI VRYDDEGGGE661EDTEAFDMAA LRNLNVIRDT KTRRDVTPEI QFLSRPAFKS IPDNVIFREF IWERLKEADV721DPGAPPYDSL QTYAFEGNGS VAESLSSLDS ISSNSDQNYD YLSDWGPRFK RLADMYGTGQ781ESLYSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
785 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.