Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDH4 / R Cadherin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99568-100
Catalog Number: LS-G99568
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human R Cadherin(CDH4) (NM_001794), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAD4 | CDH4 | Cadherin-4 | R-CAD | RCAD | Retinal cadherin | Retina cadherin | R Cadherin | R-cadherin
- NCBI GenBank
- NM_001794
- SwissProt
- P55283
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 100.28 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTAGAGVLLL LLSLSGALRA HNEDLTTRET CKAGFSEDDY TALISQNILE GEKLLQVKFS61SCVGTKGTQY ETNSMDFKVG ADGTVFATRE LQVPSEQVAF TVTAWDSQTA EKWDAVVRLL121VAQTSSPHSG HKPQKGKKVV ALDPSPPPKD TLLPWPQHQN ANGLRRRKRD WVIPPINVPE181NSRGPFPQQL VRIRSDKDND IPIRYSITGV GADQPPMEVF SIDSMSGRMY VTRPMDREEH241ASYHLRAHAV DMNGNKVENP IDLYIYVIDM NDNRPEFINQ VYNGSVDEGS KPGTYVMTVT301ANDADDSTTA NGMVRYRIVT QTPQSPSQNM FTINSETGDI VTVAAGLDRE KVQQYTVIVQ361ATDMEGNLNY GLSNTATAII TVTDVNDNPP EFTASTFAGE VPENRVETVV ANLTVMDRDQ421PHSPNWNAVY RIISGDPSGH FSVRTDPVTN EGMVTVVKAV DYELNRAFML TVMVSNQAPL481ASGIQMSFQS TAGVTISIMD INEAPYFPSN HKLIRLEEGV PPGTVLTTFS AVDPDRFMQQ541AVRYSKLSDP ASWLHINATN GQITTAAVLD RESLYTKNNV YEATFLAADN GIPPASGTGT601LQIYLIDIND NAPELLPKEA QICEKPNLNA INITAADADV DPNIGPYVFE LPFVPAAVRK661NWTITRLNGD YAQLSLRILY LEAGMYDVPI IVTDSGNPPL SNTSIIKVKV CPCDDNGDCT721TIGAVAAAGL GTGAIVAILI CILILLTMVL LFVMWMKRRE KERHTKQLLI DPEDDVRDNI781LKYDEEGGGE EDQDYDLSQL QQPEAMGHVP SKAPGVRRVD ERPVGAEPQY PIRPMVPHPG841DIGDFINEGL RAADNDPTAP PYDSLLVFDY EGSGSTAGSV SSLNSSSSGD QDYDYLNDWG901PRFKKLADMY GGGEEDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
916 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.