Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDH26 / Cadherin 26 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104814-100
Catalog Number: LS-G104814
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cadherin like 26(CDH26) (NM_177980), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cadherin-like protein 26 | CDH26 | VR20 | Cadherin 26 | Cadherin-like 26 | Cadherin-like protein VR20
- Origin Species
- Human
- NCBI GenBank
- AF169690
- SwissProt
- Q8IXH8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAMRSGRHPS LLLLLVLLLW LLQVSIIDSV QQETDDLTKQ TKEKIYQPLR RSKRRWVITT61LELEEEDPGP FPKLIGELFN NMSYNMSLMY LISGPGVDEY PEIGLFSLED HENGRIYVHR121PVDREMTPSF TVYFDVVERS TGKIVDTSLI FNIRISDVND HAPQFPEKEF NITVQENQSA181GQPIFQMLAV DLDEENTPNS QVLYFLISQT PLLKESGFRV DRLSGEIRLS GCLDYETAPQ241FTLLIRARDC GEPSLSSTTT VHVDVQEGNN HRPAFTQENY KVQIPEGRAS QGVLRLLVQD301RDSPFTSAWR AKFNILHGNE EGHFDISTDP ETNEGILNVI KPLDYETRPA QSLIIVVENE361ERLVFCERGK LQPPRKAAAS ATVSVQVTDA NDPPAFHPQS FIVNKEEGAR PGTLLGTFNA421MDPDSQIRYE LVHDPANWVS VDKNSGVVIT VEPIDRESPH VNNSFYVIII HAVDDGFPPQ481TATGTLMLFL SDINDNVPTL RPRSRYMEVC ESAVHEPLHI EAEDPDLEPF SDPFTFELDN541TWGNAEDTWK LGRNWGQSVE LLTLRSLPRG NYLVPLFIGD KQGLSQKQTV HVRICPCASG601LTCVELADAE VGLHVGALFP VCAAFVALAV ALLFLLRCYF VLEPKRHGCS VSNDEGHQTL661VMYNAESKGT SAQTWSDVEG QRPALLICTA AAGPTQGVKD LEEVPPSAAS QSAQARCALG721SWGYGKPFEP RSVKNIHSTP AYPDATMHRQ LLAPVEGRMA ETLNQKLHVA NVLEDDPGYL781PHVYSEEGEC GGAPSLSSLA SLEQELQPDL LDSLGSKATP FEEIYSESGV PSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
832 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.