Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDH24 / EY Cadherin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110343-100
Catalog Number: LS-G110343
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cadherin 24(CDH24) (NM_022478), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cadherin 24 | type 2 | CDH24 | Cadherin-24 | CDH11L | Cadherin-like 24
- Origin Species
- Human
- NCBI GenBank
- NM_022478
- SwissProt
- Q86UP0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWGLVRLLLA WLGGWGCMGR LAAPARAWAG SREHPGPALL RTRRSWVWNQ FFVIEEYAGP61EPVLIGKLHS DVDRGEGRTK YLLTGEGAGT VFVIDEATGN IHVTKSLDRE EKAQYVLLAQ121AVDRASNRPL EPPSEFIIKV QDINDNPPIF PLGPYHATVP EMSNVGTSVI QVTAHDADDP181SYGNSAKLVY TVLDGLPFFS VDPQTGVVRT AIPNMDRETQ EEFLVVIQAK DMGGHMGGLS241GSTTVTVTLS DVNDNPPKFP QSLYQFSVVE TAGPGTLVGR LRAQDPDLGD NALMAYSILD301GEGSEAFSIS TDLQGRDGLL TVRKPLDFES QRSYSFRVEA TNTLIDPAYL RRGPFKDVAS361VRVAVQDAPE PPAFTQAAYH LTVPENKAPG TLVGQISAAD LDSPASPIRY SILPHSDPER421CFSIQPEEGT IHTAAPLDRE ARAWHNLTVL ATELGWSWGP ERGWVPLLVA EWSAPAAPPQ481RSPVGSAVGI PQDSSAQASR VQVAIQTLDE NDNAPQLAEP YDTFVCDSAA PGQLIQVIRA541LDRDEVGNSS HVSFQGPLGP DANFTVQDNR DGSASLLLPS RPAPPRHAPY LVPIELWDWG601QPALSSTATV TVSVCRCQPD GSVASCWPEA HLSAAGLSTG ALLAIITCVG ALLALVVLFV661ALRRQKQEAL MVLEEEDVRE NIITYDDEGG GEEDTEAFDI TALQNPDGAA PPAPGPPARR721DVLPRARVSR QPRPPGPADV AQLLALRLRE ADEDPGVPPY DSVQVYGYEG RGSSCGSLSS781LGSGSEAGGA PGPAEPLDDW GPLFRTLAEL YGAKEPPAPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
819 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.