Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDH20 / Cadherin 20 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102010-100
Catalog Number: LS-G102010
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDH20 (NM_031891), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cadherin 20 | type 2 | CDH7L3 | Cadherin-20 | CDH20
- Origin Species
- Human
- NCBI GenBank
- NM_031891
- SwissProt
- Q9HBT6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 85.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWTSGRMSNA KNWLGLGMSL YFWGLMDLTT TVLSDTPTPQ GELEALLSDK PQSHQRTKRS61WVWNQFFVLE EYTGTDPLYV GKLHSDMDRG DGSIKYILSG EGAGIVFTID DTTGDIHAIQ121RLDREERAQY TLRAQALDRR TGRPMEPESE FIIKIQDIND NEPKFLDGPY VATVPEMSPV181GTSVIQVTAT DADDPTYGNS ARVVYSILQG QPYFSVDSKT GVIRTALMNM DREAKEYYEV241IIQAKDMGGQ LGGLAGTTTV NITLSDVNDN PPRFPQKHYQ MSVLESAPIS STVGRVFAKD301LDEGINAEMK YTIVDGDGAD AFDISTDPNF QVGIITVKKP LSFESKKSYT LKVEGANPHL361EMRFLNLGPF QDTTTVHISV EDVDEPPVFE PGFYFVEVPE DVAIGTTIQI ISAKDPDVTN421NSIRYSIDRS SDPGRFFYVD ITTGALMTAR PLDREEFSWH NITVLAMEMN NPSQVGSVPV481TIKVLDVNDN APEFPRFYEA FVCENAKAGQ LIQTVSAVDQ DDPRNGQHFY YSLAPEAANN541PNFTIRDNQD NTARILTRRS GFRQQEQSVF HLPILIADSG QPVLSSTGTL TIQVCSCDDD601GHVMSCSPEA YMLPVSLSRG ALIAILACIF VLLVLVLLIL SMRRHRKQPY IIDDEENIHE661NIVRYDDEGG GEEDTEAFDI AAMWNPREAQ AGAAPKTRQD MLPEIESLSR YVPQTCAVNS721TVHSYVLAKL YEADMDLWAP PFDSLQTYMF EGDGSVAGSL SSLQSATSDS EQSFDFLTDW781GPRFRKLAEL YGASEGPAPL WTRTRPLEQKLISEEDLAANDILDYKDDDDKV
801 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.