Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDH18 / Ey-Cadherin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130031-100
Catalog Number: LS-G130031
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDH18 (NM_001167667), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cadherin 14-like | Cadherin-14 | CDH14L | Cadherin 18 | type 2 | Cadherin-18 | CDH14 | CDH18 | EY-CADHERIN | Cadherin-like 24
- Origin Species
- Human
- NCBI GenBank
- NM_004934
- SwissProt
- Q13634
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKITSTSCIC PVLVCLCFVQ RCYGTAHHSS IKVMRNQTKH IEGETEVHHR PKRGWVWNQF61FVLEEHMGPD PQYVGKLHSN SDKGDGSVKY ILTGEGAGTI FIIDDTTGDI HSTKSLDREQ121KTHYVLHAQA IDRRTNKPLE PESEFIIKVQ DINDNAPKFT DGPYIVTVPE MSDMGTSVLQ181VTATDADDPT YGNSARVVYS ILQGQPYFSV DPKTGVIRTA LHNMDREARE HYSVVIQAKD241MAGQVGGLSG STTVNITLTD VNDNPPRFPQ KHYQLYVPES AQVGSAVGKI KANDADTGSN301ADMTYSIING DGMGIFSIST DKETREGILS LKKPLNYEKK KSYTLNIEGA NTHLDFRFSH361LGPFKDATML KIIVGDVDEP PLFSMPSYLM EVYENAKIGT VVGTVLAQDP DSTNSLVRYF421INYNVEDDRF FNIDANTGTI RTTKVLDREE TPWYNITVTA SEIDNPDLLS HVTVGIRVLD481VNDNPPELAR EYDIIVCENS KPGQVIHTIS ATDKDDFANG PRFNFFLDER LPVNPNFTLK541DNEAAAPSPS GFVHAREMGV CGPAMQKPSC PRLVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
574 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.