Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDH17 / Cadherin 17 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116904-100
Catalog Number: LS-G116904
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LI Cadherin(CDH17) (NM_004063), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cadherin 16 | Cadherin-16 | CDH17 | HPT1 | Human peptide transporter 1 | LI cadherin | HPT-1 | HPT-1 cadherin | BILL-cadherin | Cadherin | Cadherin-17 | LI-cadherin | Liver-intestine cadherin
- Origin Species
- Human
- NCBI GenBank
- NM_004063
- SwissProt
- Q12864
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.15 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MILQAHLHSL CLLMLYLATG YGQEGKFSGP LKPMTFSIYE GQEPSQIIFQ FKANPPAVTF61ELTGETDNIF VIEREGLLYY NRALDRETRS THNLQVAALD ANGIIVEGPV PITIKVKDIN121DNRPTFLQSK YEGSVRQNSR PGKPFLYVNA TDLDDPATPN GQLYYQIVIQ LPMINNVMYF181QINNKTGAIS LTREGSQELN PAKNPSYNLV ISVKDMGGQS ENSFSDTTSV DIIVTENIWK241APKPVEMVEN STDPHPIKIT QVRWNDPGAQ YSLVDKEKLP RFPFSIDQEG DIYVTQPLDR301EEKDAYVFYA VAKDEYGKPL SYPLEIHVKV KDINDNPPTC PSPVTVFEVQ ENERLGNSIG361TLTAHDRDEE NTANSFLNYR IVEQTPKLPM DGLFLIQTYA GMLQLAKQSL KKQDTPQYNL421TIEVSDKDFK TLCFVQINVI DINDQIPIFE KSDYGNLTLA EDTNIGSTIL TIQATDADEP481FTGSSKILYH IIKGDSEGRL GVDTDPHTNT GYVIIKKPLD FETAAVSNIV FKAENPEPLV541FGVKYNASSF AKFTLIVTDV NEAPQFSQHV FQAKVSEDVA IGTKVGNVTA KDPEGLDISY601SLRGDTRGWL KIDHVTGEIF SVAPLDREAG SPYRVQVVAT EVGGSSLSSV SEFHLILMDV661NDNPPRLAKD YTGLFFCHPL SAPGSLIFEA TDDDQHLFRG PHFTFSLGSG SLQNDWEVSK721INGTHARLST RHTDFEERAY VVLIRINDGG RPPLEGIVSL PVTFCSCVEG SCFRPAGHQT781GIPTVGMAVG ILLTTLLVIG IILAVVFIRI KKDKGKDNVE SAQASEVKPL RSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
832 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.