Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDH12 / Cadherin 12 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116902-100
Catalog Number: LS-G116902
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDH12 (NM_004061), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Brain cadherin | Cadherin-12 | Br-cadherin | Brain-cadherin | Cadherin | neural type | 2 | CDHB | N-cadherin 2 | Neural type cadherin 2 | CDH12 | Neuronal cadherin 2
- Origin Species
- Human
- NCBI GenBank
- NM_004061
- SwissProt
- P55289
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLTRNCLSLL LWVLFDGGLL TPLQPQPQQT LATEPRENVI HLPGQRSHFQ RVKRGWVWNQ61FFVLEEYVGS EPQYVGKLHS DLDKGEGTVK YTLSGDGAGT VFTIDETTGD IHAIRSLDRE121EKPFYTLRAQ AVDIETRKPL EPESEFIIKV QDINDNEPKF LDGPYVATVP EMSPVGAYVL181QVKATDADDP TYGNSARVVY SILQGQPYFS IDPKTGVIRT ALPNMDREVK EQYQVLIQAK241DMGGQLGGLA GTTIVNITLT DVNDNPPRFP KSIFHLKVPE SSPIGSAIGR IRAVDPDFGQ301NAEIEYNIVP GDGGNLFDIV TDEDTQEGVI KLKKPLDFET KKAYTFKVEA SNLHLDHRFH361SAGPFKDTAT VKISVLDVDE PPVFSKPLYT MEVYEDTPVG TIIGAVTAQD LDVGSSAVRY421FIDWKSDGDS YFTIDGNEGT IATNELLDRE STAQYNFSII ASKVSNPLLT SKVNILINVL481DVNEFPPEIS VPYETAVCEN AKPGQIIQIV SAADRDLSPA GQQFSFRLSP EAAIKPNFTV541RDFRNNTAGI ETRRNGYSRR QQELYFLPVV IEDSSYPVQS STNTMTIRVC RCDSDGTILS601CNVEAIFLPV GLSTGALIAI LLCIVILLAI VVLYVALRRQ KKKDTLMTSK EDIRDNVIHY661DDEGGGEEDT QAFDIGALRN PKVIEENKIR RDIKPDSLCL PRQRPPMEDN TDIRDFIHQR721LQENDVDPTA PPYDSLATYA YEGSGSVAES LSSIDSLTTE ADQDYDYLTD WGPRFKVLAD781MFGEEESYNP DKVTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
794 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.