Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDH11 / Cadherin 11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99564-100
Catalog Number: LS-G99564
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OB Cadherin(CDH11) (NM_001797), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAD11 | Cadherin 11 | Cadherin-11 | CDH11 | CDHOB | OSF-4 | Osteoblast cadherin | OB | OB-cadherin | Ventral neural cadherin | Vnc
- NCBI GenBank
- NM_001797
- SwissProt
- P55287
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.98 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKENYCLQAA LVCLGMLCHS HAFAPERRGH LRPSFHGHHE KGKEGQVLQR SKRGWVWNQF61FVIEEYTGPD PVLVGRLHSD IDSGDGNIKY ILSGEGAGTI FVIDDKSGNI HATKTLDREE121RAQYTLMAQA VDRDTNRPLE PPSEFIVKVQ DINDNPPEFL HETYHANVPE RSNVGTSVIQ181VTASDADDPT YGNSAKLVYS ILEGQPYFSV EAQTGIIRTA LPNMDREAKE EYHVVIQAKD241MGGHMGGLSG TTKVMITLTD VNDNPPKFPQ SVYQMSVSEA AVPGEEVGRV KAKDPDIGEN301GLVTYNIVDG DGMESFEITT DYETQEGVIK LKKPVDFETK RAYSLKVEAA NVHIDPKFIS361NGPFKDTVTV KIAVEDADEP PMFLAPSYIH EVQENAAAGT VVGRVHAKDP DAANSPIRYS421IDRHTDLDRF FTINPEDGFI KTTKPLDREE TAWLNITVFA AEIHNRHQEA KVPVAIRVLD481VNDNAPKFAA PYEGFICESD QTKPLSNQPI VTISADDKDD TANGPRFIFS LPPEIIHNPN541FTVRDNRDNT AGVYARRGGF SRQKQDLYLL PIVISDGGIP PMSSTNTLTI KVCGCDVNGA601LLSCNAEAYI LNAGLSTGAL IAILACIVIL LVIVVLFVTL RRQKKEPLIV FEEEDVRENI661ITYDDEGGGE EDTEAFDIAT LQNPDGINGF IPRKDIKPEY QYMPRPGLRP APNSVDVDDF721INTRIQEADN DPTAPPYDSI QIYGYEGRGS VAGSLSSLES ATTDSDLDYD YLQNWGPRFK781KLADLYGSKD TFDDDSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
796 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.