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CDCP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CDCP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CDCP1 antibodies →
$725.00each
Catalog Number: LS-G101837-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDCP1 (NM_022842), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CD318 antigen | Membrane glycoprotein gp140 | SIMA135 | TRASK | CD318 | CDCP1
Origin Species
Human
NCBI GenBank
NM_022842
SwissProt
Q9H5V8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGLNCGVSI ALLGVLLLGA ARLPRGAEAF EIALPRESNI TVLIKLGTPT LLAKPCYIVI
61SKRHITMLSI KSGERIVFTF SCQSPENHFV IEIQKNIDCM SGPCPFGEVQ LQPSTSLLPT
121LNRTFIWDVK AHKSIGLELQ FSIPRLRQIG PGESCPDGVT HSISGRIDAT VVRIGTFCSN
181GTVSRIKMQE GVKMALHLPW FHPRNVSGFS IANRSSIKRL CIIESVFEGE GSATLMSANY
241PEGFPEDELM TWQFVVPAHL RASVSFLNFN LSNCERKEER VEYYIPGSTT NPEVFKLEDK
301QPGNMAGNFN LSLQGCDQDA QSPGILRLQF QVLVQHPQNE SNKIYVVDLS NERAMSLTIE
361PRPVKQSRKF VPGCFVCLES RTCSSNLTLT SGSKHKISFL CDDLTRLWMN VEKTISCTDH
421RYCQRKSYSL QVPSDILHLP VELHDFSWKL LVPKDRLSLV LVPAQKLQQH THEKPCNTSF
481SYLVASAIPS QDLYFGSFCP GGSIKQIQVK QNISVTLRTF APSFRQEASR QGLTVSFIPY
541FKEEGVFTVT PDTKSKVYLR TPNWDRGLPS LTSVSWNISV PRDQVACLTF FKERSGVVCQ
601TGRAFMIIQE QRTRAEEIFS LDEDVLPKPS FHHHSFWVNI SNCSPTSGKQ LDLLFSVTLT
661PRTVDLTVIL IAAVGGGVLL LSALGLIICC VKKKKKKTNK GPAVGIYNGN INTEMPRQPK
721KFQKGRKDND SHVYAVIEDT MVYGHLLQDS SGSFLQPEVD TYRPFQGTMG VCPPSPPTIC
781SRAPTAKLAT EEPPPRSPPE SESEPYTFSH PNNGDVSSKD TDIPLLNTQE PMEPAE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

836 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.