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CDCA7L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CDCA7L Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111571-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDCA7L (NM_018719), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDCA7L | HR1 | JPO2 | R1 | RAM2 | Protein JPO2 | Transcription factor RAM2
Origin Species
Human
NCBI GenBank
NM_018719
SwissProt
Q96GN5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELATRYQIP KEVADIFNAP SDDEEFVGFR DDVPMETLSS EESCDSFDSL ESGKQQDVRF
61HSKYFTEELR RIFIEDTDSE TEDFAGFTQS DLNGKTNPEV MVVESDLSDD GKASLVSEEE
121EDEEEDKATP RRSRSRRSSI GLRVAFQFPT KKLANKPDKN SSSEQLFSSA RLQNEKKTIL
181ERKKDCRQVI QREDSTSESE DDSRDESQES SDALLKRTMN IKENKAMLAQ LLAELNSMPD
241FFPVRTPTSA SRKKTVRRAF SEGQITRRMN PTRSARPPEK FALENFTVSA AKFAEEFYSF
301RRRKTIGGKC REYRRRHRIS SFRPVEDITE EDLENVAITV RDKIYDKVLG NTCHQCRQKT
361IDTKTVCRNQ GCCGVRGQFC GPCLRNRYGE DVRSALLDPD WVCPPCRGIC NCSYCRKRDG
421RCATGILIHL AKFYGYDNVK EYLESLQKEL VEDN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

454 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.