LSBio
CDCA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CDCA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CDCA2 antibodies →
$725.00each
Catalog Number: LS-G106195-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDCA2 (NM_152562), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDCA2 | Repo-Man
Origin Species
Human
SwissProt
Q69YH5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
112.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDANSKDKPP ETKESAMNNA GNASFILGTG KIVTPQKHAE LPPNPCTPDT FKSPLNFSTV
61TVEQLGITPE SFVRNSAGKS SSYLKKCRRR SAVGARGSPE TNHLIRFIAR QQNIKNARKS
121PLAQDSPSQG SPALYRNVNT LRERISAFQS AFHSIKENEK MTGCLEFSEA GKESEMTDLT
181RKEGLSACQQ SGFPAVLSSK RRRISYQRDS DENLTDAEGK VIGLQIFNID TDRACAVETS
241VDLSEISSKL GSTQSGFLVE ESLPLSELTE TSNALKVADC VVGKGSSDAV SPDTFTAEVS
301SDAVPDVRSP ATPACRRDLP TPKTFVLRSV LKKPSVKMCL ESLQEHCNNL YDDDGTHPSL
361ISNLPNCCKE KEAEDEENFE APAFLNMRKR KRVTFGEDLS PEVFDESLPA NTPLRKGGTP
421VCKKDFSGLS SLLLEQSPVP EPLPQPDFDD KGENLENIEP LQVSFAVLSS PNKSSISETL
481SGTDTFSSSN NHEKISSPKV GRITRTSNRR NQLVSVVEES VCNLLNTEVQ PCKEKKINRR
541KSQETKCTKR ALPKKSQVLK SCRKKKGKGK KSVQKSLYGE RDIASKKPLL SPIPELPEVP
601EMTPSIPSIR RLGSGYFSSN GKLEEVKTPK NPVKRKDLLR HDPDLHMHQG YDKYDVSEFC
661SYIKSSSSLG NATSDEDPNT NIMNINENKN IPKAKNKSES ENEPKAGTDS PVSCASVTEE
721RVASDSPKPA LTLQQGQEFS AGGQNAENLC QFFKISPDLN IKCERKDDFL GAAEGKLQCN
781RLMPNSQKDC HCLGDVLIEN TKESKSQSED LGRKPMESSS VVSCRDRKDR RRSMCYSDGR
841SLHLEKNGNH TPSSSVGSSV EISLENSELF KDLSDAIEQT FQRRNSETKV RRSTRLQKDL
901ENEGLVWISL PLPSTSQKAK RRTICTFDSS GFESMSPIKE TVSSRQKPQM APPVSDPENS
961QGPAAGSSDE PGKRRKSFCI STLANTKATS QFKGYRRRSS LNGKGESSLT ALERIEHNGE
1021RKQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1023 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.