Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDC42EP4 / BORG4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114636-100
Catalog Number: LS-G114636
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDC42EP4 (NM_012121), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CEP4 | Binder of Rho GTPases 4 | CDC42EP4 | KAIA1777 | BORG4 | Cdc42 effector protein 4
- Origin Species
- Human
- NCBI GenBank
- NM_012121
- SwissProt
- Q9H3Q1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPILKQLVSS SVHSKRRSRA DLTAEMISAP LGDFRHTMHV GRAGDAFGDT SFLNSKAGEP61DGESLDEQPS SSSSKRSLLS RKFRGSKRSQ SVTRGEREQR DMLGSLRDSA LFVKNAMSLP121QLNEKEAAEK GTSKLPKSLS SSPVKKANDG EGGDEEAGTE EAVPRRNGAA GPHSPDPLLD181EQAFGDLTDL PVVPKATYGL KHAESIMSFH IDLGPSMLGD VLSIMDKEEW DPEEGEGGYH241GDEGAAGTIT QAPPYAVAAP PLARQEGKAG PDLPSLPSHA LEDEGWAAAA PSPGSARSMG301SHTTRDSSSL SSCTSGILEE RSPAFRGPDR ARAAVSRQPD KEFSFMDEEE EDEIRVTRTR361PLEQKLISEE DLAANDILDY KDDDDKV
387 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.