Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDC42EP1 / CEP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106396-100
Catalog Number: LS-G106396
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDC42EP1 (NM_152243), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BORG5 | CDC42EP1 | Binder of Rho GTPases 5 | CEP1 | Cdc42 effector protein 1 | MSE55 | Serum protein MSE55 | Serum constituent protein
- Origin Species
- Human
- NCBI GenBank
- NM_007061
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPGPQGGRGA ATMSLGKLSP VGWVSSSQGK RRLTADMISH PLGDFRHTMH VGRGGDVFGD61TSFLSNHGGS SGSTHRSPRS FLAKKLQLVR RVGAPPRRMA SPPAPSPAPP AISPIIKNAI121SLPQLNQAAY DSLVVGKLSF DSSPTSSTDG HSSYGLDSGF CTISRLPRSE KPHDRDRDGS181FPSEPGLRRS DSLLSFRLDL DLGPSLLSEL LGVMSLPEAP AAETPAPAAN PPAPTANPTG241PAANPPATTA NPPAPAANPS APAATPTGPA ANPPAPAASS TPHGHCPNGV TAGLGPVAEV301KSSPVGGGPR GPAGPALGRH WGAGWDGGHH YPEMDARQER VEVLPQARAS WESLDEEWRA361PQAGSRTPVP STVQANTFEF ADAEEDDEVK VTRTRPLEQKLISEEDLAANDILDYKDDDDKV
391 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.