Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDC42BPA / MRCK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113697-100
Catalog Number: LS-G113697
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDC42 binding protein kinase alpha(CDC42BPA) (NM_014826), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDC42-binding kinase alpha | CDC42BPA | DMPK-like alpha | MRCKA | MRCKalpha | KIAA0451 | MRCK | PK428 | Ser-thr protein kinase PK428 | MRCK alpha
- Origin Species
- Human
- NCBI GenBank
- NM_003607
- SwissProt
- Q5VT25
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 199.86 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGEVRLRQL EQFILDGPAQ TNGQCFSVET LLDILICLYD ECNNSPLRRE KNILEYLEWA61KPFTSKVKQM RLHREDFEIL KVIGRGAFGE VAVVKLKNAD KVFAMKILNK WEMLKRAETA121CFREERDVLV NGDNKWITTL HYAFQDDNNL YLVMDYYVGG DLLTLLSKFE DRLPEDMARF181YLAEMVIAID SVHQLHYVHR DIKPDNILMD MNGHIRLADF GSCLKLMEDG TVQSSVAVGT241PDYISPEILQ AMEDGKGRYG PECDWWSLGV CMYEMLYGET PFYAESLVET YGKIMNHKER301FQFPAQVTDV SENAKDLIRR LICSREHRLG QNGIEDFKKH PFFSGIDWDN IRNCEAPYIP361EVSSPTDTSN FDVDDDCLKN SETMPPPTHT AFSGHHLPFV GFTYTSSCVL SDRSCLRVTA421GPTSLDLDVN VQRTLDNNLA TEAYERRIKR LEQEKLELSR KLQESTQTVQ ALQYSTVDGP481LTASKDLEIK NLKEEIEKLR KQVTESSHLE QQLEEANAVR QELDDAFRQI KAYEKQIKTL541QQEREDLNKE LVQASERLKN QSKELKDAHC QRKLAMQEFM EINERLTELH TQKQKLARHV601RDKEEEVDLV MQKVESLRQE LRRTERAKKE LEVHTEALAA EASKDRKLRE QSEHYSKQLE661NELEGLKQKQ ISYSPGVCSI EHQQEITKLK TDLEKKSIFY EEELSKREGI HANEIKNLKK721ELHDSEGQQL ALNKEIMILK DKLEKTRRES QSEREEFESE FKQQYEREKV LLTEENKKLT781SELDKLTTLY ENLSIHNQQL EEEVKDLADK KESVAHWEAQ ITEIIQWVSD EKDARGYLQA841LASKMTEELE ALRNSSLGTR ATDMPWKMRR FAKLDMSARL ELQSALDAEI RAKQAIQEEL901NKVKASNIIT ECKLKDSEKK NLELLSEIEQ LIKDTEELRS EKGIEHQDSQ HSFLAFLNTP961TDALDQFETD PVENTYVWNP SVKFHIQSRS TSPSTSSEAE PVKTVDSTPL SVHTPTLRKK1021GCPGSTGFPP KRKTHQFFVK SFTTPTKCHQ CTSLMVGLIR QGCSCEVCGF SCHITCVNKA1081PTTCPVPPEQ TKGPLGIDPQ KGIGTAYEGH VRIPKPAGVK KGWQRALAIV CDFKLFLYDI1141AEGKASQPSV VISQVIDMRD EEFSVSSVLA SDVIHASRKD IPCIFRVTAS QLSASNNKCS1201ILMLADTENE KNKWVGVLSE LHKILKKNKF RDRSVYVPKE AYDSTLPLIK TTQAAAIIDH1261ERIALGNEEG LFVVHVTKDE IIRVGDNKKI HQIELIPNDQ LVAVISGRNR HVRLFPMSAL1321DGRETDFYKL SETKGCQTXT SGKVRHGALT CLCVAMKRQV LCYELFQSKT RHRKFKEIQV1381PYNVQWMAIF SEQLCVGFQS GFLRYPLNGE GNPYSMLHSN DHTLSFIAHQ PMDAICAVEI1441SSKEYLLCFN SIGIYTDCQG RRSRQQELMW PANPSSCCYN APYLSVYSEN AVDIFDVNSM1501EWIQTLPLKK VRPLNNEGSL NLLGLETIRL IYFKNKMAEG DELVVPETSD NSRKQMVRNI1561NNKRRYSFRV PEEERMQQRR EMLRDPEMRN KLISNPTNFN HIAHMGPGDG IQILKDLPMN1621PRPQESRTVF SGSVSIPSIT KSRPEPGRSM SASSGLSARS SAQNGSALKR EFSGGSYSAK1681RQPMPSPSEG SLSSGGMDQG SDAPARDFDG EDSDSPRHST ASNSSNLSSP PSPVSPRKTK1741SLSLESTDRG SWDPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1754 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.