LSBio
CDC20B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CDC20B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CDC20B antibodies →
$504.00each
Catalog Number: LS-G129951-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDC20B (NM_001170402), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDC20-like protein | CDC20B | G6VTS76519
Origin Species
Human
SwissProt
Q86Y33

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEWKLERTAP RRVRTEEEML WESIMRVLSK DLKQKRSQDS ANVLDSVNAT YSDFKSNFAK
61RLSAEVPVAS SPITTRWQQS QTRALSSDSF GEEQSTTYLP EASGSVLKTP PEKETLTLGS
121RKEQLKTPSK GISETSNSAL HFCKAPHAMD RDWKESVASK GQKCLKQLFV TQNVVQQANG
181KMQLCEQSEC VWKGCKDGVR DESFHLKSSG DINDSILQPE VKIHITGLRN DYYLNILDWS
241FQNLVAIALG SAVYIWNGEN HNGIENIDLS LTCNYISSVS WIKEGTCLAV GTSEGEVQLW
301DVVTKKRLRN MLGHLSVVGA LSWNHFILSS GSRLGRVYHH DVRVAQHHVG TLRHKQAVCA
361LKWSPDGRLL SSGCSDGLLT IWPHDPGASA QGQPLKVITQ STAVKAMDWC PWQSGVLAIG
421GGMKDGRLHI LDINAGKSIQ TPSTNSQICS LIWLPKTKEI ATGQGTPKND VTVWTCPTVS
481RSGGFFGHRG RVLHLSLSPD QTRVFSAAAD GTASVWNCY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

519 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.