LSBio
CDC14B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CDC14B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CDC14B antibodies →
$725.00each
Catalog Number: LS-G119942-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDC14B (NM_001077181), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDC14 homolog B | CDC14B | Cdc14B1 | Cdc14B2 | Cell division cycle 14b | CDC14B3 | HCDC14B
Origin Species
Human
NCBI GenBank
NM_003671
SwissProt
O60729

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSREGAGAAL VAEVIKDRLC FAILYSRPKS ASNVHYFSID NELEYENFYA DFGPLNLAMV
61YRYCCKINKK LKSITMLRKK IVHFTGSDQR KQANAAFLVG CYMVIYLGRT PEEAYRILIF
121GETSYIPFRD AAYGSCNFYI TLLDCFHAVK KAMQYGFLNF NSFNLDEYEH YEKAENGDLN
181WIIPDRFIAF CGPHSRARLE SGYHQHSPET YIQYFKNHNV TTIIRLNKRM YDAKRFTDAG
241FDHHDLFFAD GSTPTDAIVK EFLDICENAE GAIAVHCKAG LGRTGTLIAC YIMKHYRMTA
301AETIAWVRIC RPGSVIGPQQ QFLVMKQTNL WLEGDYFRQK LKGQENGQHR AAFSKLLSGV
361DDISINGVEN QDQQEPEPYS DDDEINGVTQ GDRLRALKSR RQSKTNAIPL TVILQSSVQS
421CKTSEPNISG SAGITKRTTR SASRKSSVKS LSISRTKTVL R
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

461 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.