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CDADC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CDADC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109361-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDADC1 (NM_030911), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA103J18.1 | CDADC1 | NYD-SP15 | Protein kinase NYD-SP15 | RP11-103J18.2
Origin Species
Human
NCBI GenBank
NM_030911
SwissProt
Q9BWV3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKEAGQMQNL ESARAGRSVS TQTGSMTGQI PRLSKVNLFT LLSLWMELFP AEAQRQKSQK
61NEEGKHGPLG DNEERTRVST DKRQVKRTGL VVVKNMKIVG LHCSSEDLHA GQIALIKHGS
121RLKNCDLYFS RKPCSACLKM IVNAGVNRIS YWPADPEISL LTEASSSEDA KLDAKAVERL
181KSNSRAHVCV LLQPLVCYMV QFVEETSYKC DFIQKITKTL PDANTDFYYE CKQERIKEYE
241MLFLVSNEEM HKQILMTIGL ENLCENPYFS NLRQNMKDLI LLLATVASSV PNFKHFGFYR
301SNPEQINEIH NQSLPQEIAR HCMVQARLLA YRTEDHKTGV GAVIWAEGKS RSCDGTGAMY
361FVGCGYNAFP VGSEYADFPH MDDKQKDREI RKFRYIIHAE QNALTFRCQE IKPEERSMIF
421VTKCPCDECV PLIKGAGIKQ IYAGDVDVGK KKADISYMRF GELEGVSKFT WQLNPSGAYG
481LEQNEPERRE NGVLRPVPQK EEQHQDKKLR LGIH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

514 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.