Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CD96 / TACTILE Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115714-100
Catalog Number: LS-G115714
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CD96 (NM_005816), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD96 molecule | CD96 | CD96 antigen | Cell surface antigen CD96 | T-cell surface protein tactile | TACTILE | T-Cell Activation Antigen | Increased Late Expression
- Origin Species
- Human
- NCBI GenBank
- NM_005816
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.89 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEKKWKYCAV YYIIQIHFVK GVWEKTVNTE ENVYATLGSD VNLTCQTQTV GFFVQMQWSK61VTNKIDLIAV YHPQYGFYCA YGRPCESLVT FTETPENGSK WTLHLRNMSC SVSGRYECML121VLYPEGIQTK IYNLLIQTHV TADEWNSNHT IEIEINQTLE IPCFQNSSSK ISSEFTYAWS181VEDNGTQETL ISQNHLISNS TLLKDRVKLG TDYRLHLSPV QIFDDGRKFS CHIRVGPNKI241LRSSTTVKVF AKPEIPVIVE NNSTDVLVER RFTCLLKNVF PKANITWFID GSFLHDEKEG301IYITNEERKG KDGFLELKSV LTRVHSNKPA QSDNLTIWCM ALSPVPGNKV WNISSEKITF361LLGSEISSTD PPLSVTESTL DTQPSPASSV SPARYPATSS VTLVDVSALR PNTTPQPSNS421SMTTRGFNYP WTSSGTDTKK SVSRIPSETY SSSPSGAGST LHDNVFTSTA RAFSEVPTTA481NGSTKTNHVH ITGIVVNKPK DGMSWPVIVA ALLFCCMILF GLGVRKWCQY QKEIMERPPP541FKPPPPPIKY TCIQEPNESD LPYHEMETLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
569 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.