Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CD200R1 / CD200R Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107202-100
Catalog Number: LS-G107202
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CD200R(CD200R1) (NM_138806), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD200R1 | CD200 receptor 1 | CD200R | MOX2R | OX2R | CRTR2 | MOX2 receptor
- Origin Species
- Human
- SwissProt
- Q8TD46
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLCPWRTANL GLLLILTIFL VAEAEGAAQP NNSLMLQTSK ENHALASSSL CMDEKQITQN61YSKVLAEVNT SWPVKMATNA VLCCPPIALR NLIIITWEII LRGQPSCTKA YKKETNETKE121TNCTDERITW VSRPDQNSDL QIRTVAITHD GYYRCIMVTP DGNFHRGYHL QVLVTPEVTL181FQNRNRTAVC KAVAGKPAAH ISWIPEGDCA TKQEYWSNGT VTVKSTCHWE VHNVSTVTCH241VSHLTGNKSL YIELLPVPGA KKSAKLYIPY IILTIIILTI VGFIWLLKVN GCRKYKLNKT301ESTPVVEEDE MQPYASYTEK NNPLYDTTNK VKASEALQSE VDTDLHTLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
348 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.