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CCT8L2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCT8L2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113964-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CESK1(CCT8L2) (NM_014406), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCT8L2 | CESK1 | KCNMB3L | KCNMBLP | T-complex protein 1
Origin Species
Human
NCBI GenBank
NM_014406
SwissProt
Q96SF2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSTVPSALE LPQRLALNPR ESPRSPEEEE PHLLSSLAAV QTLASVIRPC YGPHGRQKFL
61VTMKGETVCT GCATAILRAL ELEHPAAWLL REAGQTQAEN SGDGTAFVVL LTEALLEQAE
121QLLKAGLPRP QLREAYATAT AEVLATLPSL AIQSLGPLED PSWALHSVMN THTLSPMDHL
181TKLVAHACWA IKELDGSFKP ERVGVCALPG GTLEDSCLLP GLAISGKLCG QMATVLSGAR
241VALFACPFGP AHPNAPATAR LSSPADLAQF SKGSDQLLEK QVGQLAAAGI NVAVVLGEVD
301EETLTLADKY GIVVIQARSW MEIIYLSEVL DTPLLPRLLP PQRPGKCQRV YRQELGDGLA
361VVFEWECTGT PALTVVLRGA TTQGLRSAEQ AVYHGIDAYF QLCQDPRLIP GAGATEMALA
421KMLSDKGSRL EGPSGPAFLA FAWALKYLPK TLAENAGLAV SDVMAEMSGV HQGGNLLMGV
481GTEGIINVAQ EGVWDTLIVK AQGFRAVAEV VLQLVTVDEI VVAKKSPTHQ EIWNPDSKKT
541KKHPPPVETK KILGLNN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

557 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.