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CCT6A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCT6A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCT6A antibodies →
$725.00each
Catalog Number: LS-G118411-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCT6A (NM_001762), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCT-zeta-1 | Cctz | CCT-zeta | CCT6 | CCT6A | HTR3 | TCP-1-zeta | TCP20 | TCPZ | MoDP-2 | TTCP20
Origin Species
Human
NCBI GenBank
NM_001762
SwissProt
P40227

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAVKTLNPK AEVARAQAAL AVNISAARGL QDVLRTNLGP KGTMKMLVSG AGDIKLTKDG
61NVLLHEMQIQ HPTASLIAKV ATAQDDITGD GTTSNVLIIG ELLKQADLYI SEGLHPRIIT
121EGFEAAKEKA LQFLEEVKVS REMDRETLID VARTSLRTKV HAELADVLTE AVVDSILAIK
181KQDEPIDLFM IEIMEMKHKS ETDTSLIRGL VLDHGARHPD MKKRVEDAYI LTCNVSLEYE
241KTEVNSGFFY KSAEEREKLV KAERKFIEDR VKKIIELKRK VCGDSDKGFV VINQKGIDPF
301SLDALSKEGI VALRRAKRRN MERLTLACGG VALNSFDDLS PDCLGHAGLV YEYTLGEEKF
361TFIEKCNNPR SVTLLIKGPN KHTLTQIKDA VRDGLRAVKN AIDDGCVVPG AGAVEVAMAE
421ALIKHKPSVK GRAQLGVQAF ADALLIIPKV LAQNSGFDLQ ETLVKIQAEH SESGQLVGVD
481LNTGEPMVAA EVGVWDNYCV KKQLLHSCTV IATNILLVDE IMRAGMSSLK G
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

531 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.