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CCT3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCT3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCT3 antibodies →
$725.00each
Catalog Number: LS-G121883-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCT3 (NM_001008800), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCT-gamma | CCTG | HTRiC5 | TCP-1-gamma | TRIC5 | CCT3 | PIG48
Origin Species
Human
NCBI GenBank
NM_005998
SwissProt
P49368

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMGHRPVLVL SQNTKRESGR KVQSGNINAA KIQVQHPAAK SMIEISRTQD EEVGDGTTSV
61IILAGEMLSV AEHFLEQQMH PTVVISAYRK ALDDMISTLK KISIPVDISD SDMMLNIINS
121SITTKAISRW SSLACNIALD AVKMVQFEEN GRKEIDIKKY ARVEKIPGGI IEDSCVLRGV
181MINKDVTHPR MRRYIKNPRI VLLDSSLEYK KGESQTDIEI TREEDFTRIL QMEEEYIQQL
241CEDIIQLKPD VVITEKGISD LAQHYLMRAN ITAIRRVRKT DNNRIARACG ARIVSRPEEL
301REDDVGTGAG LLEIKKIGDE YFTFITDCKD PKACTILLRG ASKEILSEVE RNLQDAMQVC
361RNVLLDPQLV PGGGASEMAV AHALTEKSKA MTGVEQWPYR AVAQALEVIP RTLIQNCGAS
421TIRLLTSLRA KHTQENCETW GVNGETGTLV DMKELGIWEP LAVKLQTYKT AVETAVLLLR
481IDDIVSGHKK KGDDQSRQGG APDAGQE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

507 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.