Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCPG1 / CRP8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111047-100
Catalog Number: LS-G111047
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCPG1 (NM_020739), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCP8 | Cell cycle progression 1 | CPR8 | CCPG1 | KIAA1254
- Origin Species
- Human
- NCBI GenBank
- NM_004748
- SwissProt
- Q9ULG6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSENSSDSDS SCGWTVISHE GSDIEMLNSV TPTDSCEPAP ECSSLEQEEL QALQIEQGES61SQNGTVLMEE TAYPALEETS STIEAEEQKI PEDSIYIGTA SDDSDIVTLE PPKLEEIGNQ121EVVIVEEAQS SEDFNMGSSS SSQYTFCQPE TVFSSQPSDD ESSSDETSNQ PSPAFRRRRA181RKKTVSASES EDRLVGEQET EPSKELSKRQ FSSGLNKCVI LALVIAISMG FGHFYGTIQI241QKRQQLVRKI HEDELNDMKD YLSQCQQEQE SFIDYKSLKE NLARCWTLTE AEKMSFETQK301TNLATENQYL RVSLEKEEKA LSSLQEELNK LREQIRILED KGTSTELVKE NQKLKQHLEE361EKQKKHSFLS QRETLLTEAK MLKRELERER LVTTALRGEL QQLSGSQLHG KSDSPNVYTE421KKEIAILRER LTELERKLTF EQQRSDLWER LYVEAKDQNG KQGTDGKKKG GRGSHRAKNK481SKETFLGSVK ETFDAMKNST KEFVRHHKEK IKQAKEAVKE NLKKFSDSVK STFRHFKDTT541KNIFDEKGNK RFGATKEAAE KPRTVFSDYL HPQYKAPTEN HHNRGPTMQN DGRKEKPVHF601KEFRKNTNSK KCSPGHDCRE NSHSFRKACS GVFDCAQQES MSLFNTVVNP IRMDEFRQII661QRYMLKELDT FCHWNELDQF INKFFLNGVF IHDQKLFTDF VNDVKDYLRN MKEYEVDNDG721VFEKLDEYIY RHFFGHTFSP PYGPRSVYIK PCHYSSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
757 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.