Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCNT2 / Cyclin T2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118708-100
Catalog Number: LS-G118708
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cyclin T2(CCNT2) (NM_001241), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cyclin T2b | CYCT2 | CCNT2 | Cyclin-T2 | Cyclin T2 | Cyclin T2a
- Origin Species
- Human
- NCBI GenBank
- NM_001241
- SwissProt
- O60583
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASGRGASSR WFFTREQLEN TPSRRCGVEA DKELSCRQQA ANLIQEMGQR LNVSQLTINT61AIVYMHRFYM HHSFTKFNKN IISSTALFLA AKVEEQARKL EHVIKVAHAC LHPLEPLLDT121KCDAYLQQTQ ELVILETIML QTLGFEITIE HPHTDVVKCT QLVRASKDLA QTSYFMATNS181LHLTTFCLQY KPTVIACVCI HLACKWSNWE IPVSTDGKHW WEYVDPTVTL ELLDELTHEF241LQILEKTPNR LKKIRNWRAN QAARKPKVDG QVSETPLLGS SLVQNSILVD SVTGVPTNPS301FQKPSTSAFP APVPLNSGNI SVQDSHTSDN LSMLATGMPS TSYGLSSHQE WPQHQDSART361EQLYSQKQET SLSGSQYNIN FQQGPSISLH SGLHHRPDKI SDHSSVKQEY THKAGSSKHH421GPISTTPGII PQKMSLDKYR EKRKLETLDL DVRDHYIAAQ VEQQHKQGQS QAASSSSVTS481PIKMKIPIAN TEKYMADKKE KSGSLKLRIP IPPTDKSASK EELKMKIKVS SSERHSSSDE541GSGKSKHSSP HISRDHKEKH KEHPSSRHHT SSHKHSHSHS GSSSGGSKHS ADGIPPTVLR601SPVGLSSDGI SSSSSSSRKR LHVNDASHNH HSKMSKSSKS SGGLRTSQHP RETGQEASGD661QRSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
663 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.