Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCNO / UNG2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110743-100
Catalog Number: LS-G110743
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCNO (NM_021147), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCNU | CCNO | Cyclin domain containing | Cyclin O | Cyclin U | Uracil-DNA glycosylase 2 | UNG2 | Cyclin-O | UDG2
- Origin Species
- Human
- NCBI GenBank
- NM_021147
- SwissProt
- P22674
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVTPCPTSPS SPAARAGRRD NDQNLRAPVK KSRRPRLRRK QPLHPLNPCP LPGDSGICDL61FESPSSGSDG AESPSAARGG SPLPGPAQPV AQLDLQTFRD YGQSCYAFRK AQESHFHPRE121ALARQPQVTA ESRCKLLSWL IPVHRQFGLS FESLCLTVNT LDRFLTTTPV AADCFQLLGV181TSLLIACKQV EVHPPRVKQL LALCCGAFSR QQLCNLECIV LHKLHFTLGA PTISFFLEHF241THARVEAGQA EASEALEAQA LARGVAELSL ADYAFTSYSP SLLAICCLAL ADRMLRVSRP301VDLRLGDHPE AALEDCMGKL QLLVAINSTS LTHMLPVQIC EKCSLPPSSKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
350 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.