Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCNL2 / Cyclin L2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109346-100
Catalog Number: LS-G109346
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCNL2 (NM_030937), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ANIA-6B | CCNL2 | CCNM | Cyclin M | HLA-ISO | PCEE | SB138 | Cyclin L2 | Cyclin-L2 | HCLA-ISO
- Origin Species
- Human
- NCBI GenBank
- NM_030937
- SwissProt
- Q96S94
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAAAAAGA AGSAAPAAAA GAPGSGGAPS GSQGVLIGDR LYSGVLITLE NCLLPDDKLR61FTPSMSSGLD TDTETDLRVV GCELIQAAGI LLRLPQVAMA TGQVLFQRFF YTKSFVKHSM121EHVSMACVHL ASKIEEAPRR IRDVINVFHR LRQLRDKKKP VPLLLDQDYV NLKNQIIKAE181RRVLKELGFC VHVKHPHKII VMYLQVLECE RNQHLVQTSW NYMNDSLRTD VFVRFQPESI241ACACIYLAAR TLEIPLPNRP HWFLLFGATE EEIQEICLKI LQLYARKKVD LTHLEGEVEK301RKHAIEEAKA QARGLLPGGT QVLDGTSGFS PAPKLVESPK EGKGSKPSPL SVKNTKRRLE361GAKKAKADSP VNGLPKGRES RSRSRSREQS YSRSPSRSAS PKRRKSDSGS TSGGSKSQSR421SRSRSDSPPR QAPRSAPYKG SEIRGSRKSK DCKYPQKPHK SRSRSSSRSR SRSRERADNP481GKYKKKSHYY RDQRRERSRS YERTGRRYER DHPGHSRHRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
520 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.