Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCNI / Cyclin I Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115054-100
Catalog Number: LS-G115054
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cyclin(CCNI) (NM_006835), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCNI | Cyclin I | Cyclin-I | CYI | Cyclin ITI
- Origin Species
- Human
- NCBI GenBank
- NM_006835
- SwissProt
- Q14094
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKFPGPLENQ RLSFLLEKAI TREAQMWKVN VRKMPSNQNV SPSQRDEVIQ WLAKLKYQFN61LYPETFALAS SLLDRFLATV KAHPKYLSCI AISCFFLAAK TVEEDERIPV LKVLARDSFC121GCSSSEILRM ERIILDKLNW DLHTATPLDF LHIFHAIAVS TRPQLLFSLP KLSPSQHLAV181LTKQLLHCMA CNQLLQFRGS MLALAMVSLE MEKLIPDWLS LTIELLQKAQ MDSSQLIHCR241ELVAHHLSTL QSSLPLNSVY VYRPLKHTLV TCDKGVFRLH PSSVPGPDFS KDNSKPEVPV301RGTAAFYHHL PAASGCKQTS TKRKVEEMEV DDFYDGIKRL YNEDNVSENV GSVCGTDLSR361QEGHASPCPP LQPVSVMTRT RPLEQKLISE EDLAANDILD YKDDDDKV
408 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.