Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCL16 / LEC Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100350-100
Catalog Number: LS-G100350
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LEC(CCL16) (NM_004590), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cc chemokine lcc-1 | CCL16 | CKb12 | Chemokine hcc-4 | Chemokine LEC | Chemokine lmc | ILINCK | Liver CC chemokine-1 | Monotactin-1 | NCC-4 | LCC-1 | LEC | Small-inducible cytokine A16 | Liver-expressed chemokine | LMC | New CC chemokine 4 | C-C motif chemokine 16 | Chemokine CC-4 | Chemokine ncc-4 | HCC-4 | IL-10-inducible chemokine | Mtn-1 | NCC4 | SCYA16 | SCYL4
- Origin Species
- Hu
- NCBI GenBank
- NM_004590
- SwissProt
- O15467
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 13.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKVSEAALSL LVLILIITSA SRSQPKVPEW VNTPSTCCLK YYEKVLPRRL VVGYRKALNC61HLPAIIFVTK RNREVCTNPN DDWVQEYIKD PNLPLLPTRN LSTVKIITAK NGQPQLLNSQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
120 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.