Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCL15 / MIP5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116832-100
Catalog Number: LS-G116832
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Macrophage inflammatory protein 5(CCL15) (NM_004167), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C-C motif chemokine 15 | Chemokine hcc2 | HMRP-2B | Leukotactin-1 | LKN1 | MIP-5 | MIP5 | MRP-2B | Leukotactin1 | Small-inducible cytokine A15 | MIP-1D | NCC3 | New CC chemokine 3 | SY15 | CCL15 | Chemokine CC-2 | HCC-2 | Leukotactin 1 | LKN-1 | MIP-1 delta | NCC-3 | SCYA15
- Origin Species
- Human
- NCBI GenBank
- NM_004167
- SwissProt
- Q16663
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 12.24 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKVSVAALSC LMLVAVLGSQ AQFTNDAETE LMMSKLPLEN PVVLNSFHFA ADCCTSYISQ61SIPCSLMKSY FETSSECSKP GVIFLTKKGR QVCAKPSGPG VQDCMKKLKP YSITRTRPLEQKLISEEDLAANDILDYKDDDDKV
113 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.