Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCIN / Calicin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115671-100
Catalog Number: LS-G115671
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calicin(CCIN) (NM_005893), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCIN | BTBD20 | Calicin
- Origin Species
- Human
- NCBI GenBank
- AF333334
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLEFTEKNY NSFVLQNLNR QRKRKEYWDM ALSVDNHVFF AHRNVLAAVS PLVRSLISSN61DMKTADELFI TIDTSYLSPV TVDQLLDYFY SGKVVISEQN VEELLRGAQY FNTPRLRVHC121NDFLIKSICR ANCLRYLFLA ELFELKEVSD VAYSGIRDNF HYWASPEGSM HFMRCPPVIF181GRLLRDENLH VLNEDQALSA LINWVYFRKE DREKYFKKFF NYINLNAVSN KTLVFASNKL241VGMENTSSHT TLIESVLMDR KQERPCSLLV YQRKGALLDS VVILGGQKAH GQFNDGVFAY301IIQENLWMEL SDMPYRAAAL SATSAGRYIY ISGGTTEQIS GLKTAWRYDM DDNSWTKLPD361LPIGLVFHTM VTCGGTVYSV GGSIAPRRYV SNIYRYDERK EVWCLAGKMS IPMDGTAVIT421KGDRHLYIVT GRCLVKGYIS RVGVVDCFDT STGDVVQCIT FPIEFNHRPL LSFQQDNILC481VHSHRQSVEI NLQKVKASKT TTSVPVLPNS CPLDVSHAIC SIGDSKVFVC GGVTTASDVQ541TKDYTINPNA FLLDQKTGKW KTLAPPPEAL DCPACCLAKL PCKILQRITRTRPLEQKLISEEDLAANDILDYKDDDDKV
588 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.